LOCUS       EF1a-FLuc-T2A-RFP       6488 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
COMMENT     
FEATURES             Location/Qualifiers
     LTR             6254..6488
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    5575..6171
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             4801..4854
                     /label=T2A
     CDS             4855..5559
                     /label=RFP
     CDS             3136..4785
                     /label=Firefly\Luciferase\(FLuc)
BASE COUNT     1567 a      1694 c      1846 g      1381 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccggtt 
     3121 aacggatccg ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac 
     3181 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg 
     3241 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag 
     3301 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac 
     3361 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc 
     3421 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg 
     3481 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag 
     3541 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag 
     3601 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc 
     3661 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc 
     3721 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct 
     3781 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc 
     3841 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac 
     3901 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc 
     3961 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc 
     4021 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc 
     4081 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc 
     4141 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg 
     4201 gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac 
     4261 ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc 
     4321 atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac 
     4381 ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg 
     4441 gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag 
     4501 agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac 
     4561 gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag 
     4621 aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt 
     4681 gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc 
     4741 cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtggctag cggcgatatc 
     4801 gagggcagag gcagcctgct gacatgcgga gatgtggaag agaaccccgg gcccatggtg 
     4861 tccaagggcg aggaagtgat caaagagttc atgcggttca aagtgcggat ggaaggcagc 
     4921 atgaacggcc acgagttcga gatcgagggc gagggcgaag gcagacctta cgagggaacc 
     4981 cagaccgcca agctgaaagt gaccaagggc ggacccctgc ccttcgcctg ggatattctg 
     5041 agcccccagt ttatgtacgg cagcaaagcc tacgtgaagc accccgccga catccccgac 
     5101 tacaagaagc tgagcttccc cgagggcttc aagtgggaga gagtgatgaa cttcgaggac 
     5161 ggcggcctcg tgaccgtgac ccaggatagc tctctgcagg acggcaccct gatctacaaa 
     5221 gtgaagatgc ggggcaccaa cttccccccc gatggccccg tgatgcagaa aaagaccatg 
     5281 ggctgggagg ccagcaccga gagactgtac cctagagatg gcgtgctgaa gggcgagatc 
     5341 caccaggccc tgaagctgaa ggatggcggc cactacctgg tggagttcaa gaccatctac 
     5401 atggccaaga aacccgtgca gctgcccggc tactactacg tggacaccaa gctggacatc 
     5461 accagccaca acgaggacta caccatcgtg gaacagtacg agcggagcga gggccggcac 
     5521 cacctgtttc tgtatggcat ggacgagctg tacaagtgaa ctagtgcggc cgcgtcgaca 
     5581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     5641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     5701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     5761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     5821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     5881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     5941 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 
     6001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     6061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     6121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag 
     6181 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 
     6241 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 
     6301 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 
     6361 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 
     6421 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 
     6481 ctctagca 
//
